Cell Biology Lab

Cell Biology Lab

Content: Eukaryotic cell cultures are routinely used in biotechnological and toxicological protocols. Cells cultivated in appropriate conditions can be used for screening newly created molecules, thereby significantly reducing the number of animal tests. At the early stages of the drug development process, properly planned experiments using cell lines enable investigators to answer many questions about the mechanism of action, initial toxicological activity, or interactions with other substances.

The Cell Biology Lab uses a wide range of research models and techniques. The research methodology includes techniques of cultivating over 100 different pre-defined cell lines and original cells. Routinely used screening tests include MTT and measurement of ATP levels. The following techniques are employed to study the molecular mechanism of action: qRT-PCR, flow cytometry, confocal microscopy, Luminex, and tests of ABC transporter activity, cell migration, and tubulogenesis. Last year, CRISPR models and stable transfections were implemented.

The Lab is equipped with state-of-the-art equipment for maintaining sterility; a system of liquid nitrogen cell banks, fluorescence microscopes and Leica’s latest confocal microscope with a resonant scanner and an environmental chamber, FACS Canto II flow cytometer, Luminex Magpix, LightCycler 480 II, and multifunctional microplate readers.